Mayeso a COVID 19 Antibody (IgG)

Kukula ndi kulondola kwa kuyezetsa kachilombo ka SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) virus, chomwe chimayambitsa NKHANI 19 zikusintha pang'onopang'ono ku India, pomwe zida zatsopano ndi zotsogola zikupangidwa ndi makampani opanga sayansi padziko lonse lapansi.

Njira zitatu zomwe zimagwiritsidwa ntchito kwambiri ndi monga - Antibody Testing (IgG), Reverse Transcription Polymerase Chain Reaction (RT - PCR) ndi njira. TrueNat. Onse ndi osiyana wina ndi mzake. Ali ndi mphamvu ndi zolephera zawo, ubwino, ndi kuipa kwawo.

Real-time RT-PCR ikupitilizabe kukhala njira yodalirika komanso yolondola kwambiri mpaka pano. Komabe, palibe njira yamunthu yomwe ili yolondola 100% chifukwa cha kusintha kwa ma virus.

Kuyesa kwa Antibody (IgG)

Kuyeza ma antibodies kumadziwikanso kuti kuyesa kwa serological. Dokotala wanu kapena katswiri wa labotale yachipatala adzagwiritsa ntchito kuyesa mtundu wa ma antibodies omwe amapezeka m'magazi anu. Ma antibodies anu ndi mamolekyu a protein. Amamanga ku tinthu takunja ngati ma virus (pankhaniyi) ndikuchenjeza chitetezo chanu chamthupi kuti chichitepo kanthu.

Pali ma antibodies ambiri m'magazi. Katswiri kapena namwino atenga magazi anu ndikuwunika ngati ali ndi IgM ndi IgG. Ig imayimira molekyulu ya immunoglobulin.
● Ma antibodies a IgM amapangidwa atangoyamba kumene kutenga kachilombo ka SARS-CoV-2.
● Ma antibodies a IgG amayamba motsutsana ndi SARS-CoV-2 munthu akangochira ku coronavirus.

Ma enzyme-Linked Immunosorbent Assay (ELISA) ndi mtundu wa mayeso a antibody. Lapangidwa kuti liwonetsere dera lalikulu pakanthawi kochepa.

Zotsatira za Antibody Test (IgG)

Zida zoyezera ma antibody zimatenga pafupifupi mphindi 30-60 kuti ziwonetse zotsatira.

Ubwino Woyesa Ma Antibody (IgG)

● Kuyezetsa chitetezo cha mthupi n'kothandiza poyesa zitsanzo zambiri pakanthawi kochepa.
● Zimathandiza kuŵerengera kuchuluka kwa matenda monga mmene kachilomboka kakufalikira mofulumira.
● Ndikoyenera kuchita kafukufuku wofufuza ngati anthu ali ndi kachilomboka kapena ayi.
● Amagwiritsidwanso ntchito pofufuza zinthu zomwe zimakhudza kuopsa kwa matendawa.

Kuipa kwa Mayeso a Antibody (IgG)

● Mayesowa ali ndi malire olakwika. Ma antibody kits amatha kukupatsirani zotsatira mkati mwa mphindi 30-60 koma sizolondola kwambiri kuposa kuyesa kwa m'mphuno.
● Mayeso ozindikira kukhalapo kwa ma antibodies a IgM amatha kupereka zotsatira mkati mwa mphindi 20. Kumbali inayi, kuyezetsa kuti azindikire kukhalapo kwa ma antibodies a IgG kumatha kutenga sabata. Mayeso a IgG ndi odalirika kuposa IgM.
● Zotsatira zolakwika - pamakhala funso lotsimikizira za ubwino wa mayeso. Mayesowa sapereka kapena kutsimikizira 100% kulondola. Zida zochepa zimagwira bwino kuposa zina.
● Pali kusalondola kwakukulu pakuyesa odwala omwe alibe zizindikiro.

RT-PCR imabwera m'gulu la mayeso othamanga omwe amagwiritsidwa ntchito kwambiri ndi mayiko osiyanasiyana.

Reverse Transcript Polymerase Chain Reaction (RT - PCR)

Mayeso a polymerase chain reaction ndi mayeso ovuta kwambiri. Chifukwa chakuchulukirachulukira komanso kukhulupirika kwake, imadziwika kuti ndiyo njira yolondola kwambiri yoyesera ya COVID 19 mpaka pano. Zimagwira ntchito pozindikira kupezeka kwa chibadwa cha tizilombo toyambitsa matenda. RT-PCR idagwiritsidwa ntchito kwambiri panthawi ya kachilombo ka Ebola komanso Zika kachilombo.

Akatswiri ophunzitsidwa adzatenga swab kuchokera pamphuno kapena mmero. Idzathandizidwa ndi mankhwala angapo omwe amatha kuchotsa mapuloteni ndi mafuta kuti atulutse RNA yokhayo yomwe ilipo. RNA iyi imapangidwa kuti ipite pansi polemba kuti ipange DNA ya viral. RT-PCR yeniyeni yeniyeni imadutsa maulendo 35 ndi DNA ya tizilombo ndipo imapanga pafupifupi 35 biliyoni. Lili ndi zigawo za viral DNA. Zigawo za DNA zimatulutsa utoto wa fulorosenti ngati kachilomboka kalipo.

Zotsatira za RT-PCR

RT-PCR imatha kupereka matenda olondola komanso zotsatira za COVID 19 mkati mwa maola atatu. Ma laboratories amatenga maola 3-6 kuti apeze zotsatira zomaliza.

Ubwino wa RT-PCR

● RT–PCR ndi yachindunji ndipo imakhudzidwa kwambiri ndi kachilomboka.
● Kulondola kwake ndi mlingo wake ndi wapamwamba kuposa njira zina.
● Ndiwocheperako ku kuipitsidwa komwe kumachepetsa malire a zolakwika.
● Imatha kuzindikira kuopsa kwa matendawa.

Zoyipa za RT-PCR

● Imatha kuzindikira matenda omwe akupitirirabe. Izi zimalepheretsa madokotala kumvetsetsa kukula ndi kufalikira kwa kachilomboka. Izi zimapanga kusatsimikizika.
● Kuyesa kwa RT-PCR kumafunika zida zapadera. Sikophweka kugwira ntchito ngati kuyesa kwa antibody, komwe kumangofunika zida.
● Kugwiritsa ntchito makina onyamulika a RT-PCR kumafuna maphunziro apadera kuti apewe kugwiritsidwa ntchito molakwa kulikonse.
● Ndi okwera mtengo.

TrueNat

TrueNat ndi makina opangidwa ndi chip, osunthika a RT-PCR omwe adapangidwa kuti azindikire chifuwa chachikulu. Mutha kutsimikizira zitsanzo zanu pogwiritsa ntchito mayeso otsimikizira a SARS-CoV-2 ngati mutapezeka ndi TrueNat Beta CoV.

Zotsatira za TrueNat

Imatha kutulutsa zotsatira mwachangu kuposa mayeso wamba a RT-PCR.

Ubwino wa TrueNat

● Ndi kuyesa kwa PCR ndipo ndi kodalirika.
● Kukhudzika kwakukulu koyambira ndi tsatanetsatane
● Mapangidwe osagwirizana ndi kuipitsidwa/kutuluka kwa nthunzi
● Imathandizira kuyezetsa ndi kupereka malipoti tsiku lomwelo. Izi zimathandiza kuti odwala azidzipatula mwachangu ngati pakufunika.

Zoyipa za TrueNat

Palibe zoletsa zazikulu zomwe zanenedwa za TrueNat. TrueNat imachokera paukadaulo wa PCR ndipo idzakhala ndi zovuta zofanana ndi kuyesa kwa RT-PCR.

Kukhoza kuyesa kukupitilira kukhala chovuta kwa onse. Mayeso achangu a antibody ndi othandiza kwambiri koma sangazindikire wodwala wopanda zizindikiro. Wodwala wopanda zizindikiro sawonetsa zizindikiro zilizonse koma amatha kukupatsirani. Pakuzindikira mozama kwa SARS-CoV-2, kuyesa ngati RT-PCR ndi TrueNat ndikolondola kwambiri. Uku kunali kuyang'ana mwatsatanetsatane mitundu yosiyanasiyana ya mayeso ovomerezeka omwe akuchitika kuti azindikire COVID-19.

Kutsiliza

Mayiko ambiri padziko lonse lapansi atembenukira ku kuyesa kotengera PCR chifukwa ndi odalirika komanso othamanga kwambiri chifukwa kuyesa kwa antibody nthawi zambiri kumakhala njira yotsika mtengo, yachangu komanso yowopsa m'malo ena.

Kusiyanasiyana kwakukulu kwa njira zoyesera ndi kuchuluka kwa mayiko kumapangitsa kuti ziwerengero zapakati pa mayiko zisayerekezedwe kwenikweni. Koma m'dziko lonselo, njira zofananira ndi zida zoyeserera zofala zikutsatiridwa.

Ibibazo

Kodi kafukufuku wa UMASS COVID antibody ndi chiyani?

Anthu amene achira kwathunthu Covid 19 The UMass Memorial Medical Center, wakhala ndi wodwala kwambiri wa COVID-19 yemwe adawona kusintha kochititsa chidwi atalandira chithandizo choyamba cha plasma m'chipatala, akuganizira za chithandizo cha plasma kwa odwala omwe ali ndi vuto lalikulu kapena lomwe laika moyo pachiwopsezo. za matenda.

Chodzikanira:
Nkhaniyi ndi yongofuna kudziwa zambiri zokha ndipo sikulowa m'malo mwa malangizo achipatala. Nthawi zonse funsani dokotala wodziwa bwino zachipatala kuti mudziwe zolondola komanso malingaliro amunthu payekha.