Cudurka Molecular Pathology

Cilmi-nafsiga Molecular Pathology waa goob cilmi-nafsiyeedka oo ka faa'iidaysata mabaadi'da, farsamooyinka iyo qalabka bayoolaji molecular taas oo ah qalab aad lagama maarmaan u ah ku-dhaqanka daawada saxda ah.

Myeloid neoplasms

• Ba'an Myeloid Leukemia.
• Myeloproliferative neoplasms.
• Myelodysplastic syndromes.
• Myelodysplastic / myeloproliferative neoplasms.
• Myeloid/lymphoid neoplasms oo leh eosinophilia iyo dib u habeynta hidda-socodka.
• Mastocytosis

Lymphoid neoplasms

• B-lymphoblastic leukemia/lymphoma
• Leukemia lymphocytic chronic
• Unugyada Plasma neoplasms
• lymfoma B-cell-sare

Isticmaalka tignoolajiyada kala duwan sida PCR-waqtiga-dhabta ah, Fluorescence in situ hybridization (FISH), Falanqaynta jajabka iyo Taxanaha Jiilka Xiga (NGS) waxay caawisaa in si sax ah loo tilmaamo cilladaha hidde-sideyaasha ee keena cudurka.

• PCR waxay ogolaataa in la kordhiyo jajabka DNA-da si loo fududeeyo ogaanshaha. PCR waxaa laga yaabaa in loo isticmaalo in lagu raadiyo isbeddellada qaarkood ee hidda-sidaha ama koromosoomyada, kuwaas oo laga yaabo inay caawiyaan helitaanka iyo ogaanshaha xaalad hidde ama cudur, sida kansarka.
• Waqtiga dhabta ah PCR waxay ku bilaabataa qaabka DNA/RNA (laga soo saaray muunada bukaanka) iyo bartilmaameedyada xiisaha (tusaale JAK2 mutation ee neoplasms myeloproliferative neoplasms ama qoraalada isku dhafka BCR-ABL ee CML) ayaa la xoojiyay malaayiin jeer, kuwaas oo la ogaan karo waqtiga dhabta ah iyo si tayo leh loo sheegay (la ogaaday/aan la ogaan) ama tiro ahaan (tusaale koobiyo/ml).
• KALUUNKA – Habka cytogenetics molecular / farsamada shaybaarka ee lagu ogaanayo laguna heli karo taxane DNA gaar ah oo ku jira koromosoomyada, Halkaas oo shaybaarada lagu farsameeyo si loo fududeeyo isku xirka DNA-da bartilmaameedka ah ee DNA-da lagu calaamadiyay fluorochrome, taas oo u oggolaanaysa aragtida dambe ee muraayadda fluorescence.
• Falanqaynta jajabka: Ka dib kordhinta bartilmaameedka (PCR-ga caadiga ah), kala-soocidda iyo muuqaalaynta jajabyada sida fluorescently calaamadsan (DNA) ee ku salaysan cabbirka (tusaale Microsatellite xasilloonida).
• NGS (isku xigxiga jiilka soo socda) - Waxay suurtagelisaa ogaanshaha isku mar ah ee isbeddellada hidde-sidaha ee ogaanshaha iyo saadaalinta ku habboon ee hiddo-wadaha badan kuwaas oo daawaynta la beegsaday loo heli karo. Qiimayntu waxa ay ka bilaabataa diyaarinta maktabadda iyo qaab-dhismeedka ka soo baxana si weyn ayaa loo xoojiyey oo la tayeeyey kuwaas oo la sii taxaabay. Wax ka beddelka hidde-sidaha waxaa lagu sharraxay iyadoo la isticmaalayo software ganacsi laga heli karo kaasoo ku xiraya daawaynta la heli karo, tijaabooyinka bukaan-socodka iyo khatarta.

• Burooyinka adag:

  10ng of input nucleic acid waxa ay ogaataa bartilmaameedyada la fulin karo ee guud ahaan 52 hiddo-sideyaasha. Haddii bartilmaameed la taaban karo la aqoonsado, daawaynta la beegsanayo ayaa la bixiyaa, taas oo beegsata hiddo-sideyaal gaar ah iyo borotiinno kuwaas oo ku lug leh koritaanka iyo badbaadada unugyada kansarka iyada oo aan saameyn ku yeelan unugyada caadiga ah. Waxay ka shaqeysaa iyada oo xannibaysa calaamadaha koritaanka lagama maarmaanka u ah faafitaanka unugyada kansarka, waxay dedejisaa burburkeeda waxayna sidoo kale hoos u dhigtaa wax soo saarka xididdada dhiigga cusub ee looga baahan yahay badbaadada. Daaweyntani waxay sii dheerayn doontaa badbaadada bukaanka waxayna hagaajin doontaa natiijada guud ee bukaanka. Waxay ka caawisaa ogaanshaha unugyada jilicsan ee sarcomas iyo sidoo kale burooyinka unugyada wareegsan ee yaryar iyadoo la ogaanayo cilladda cytogenetic ee sifaha ah.

• Myeloid neoplasms:

  Isla mar ahaantaana wuxuu ogaanayaa dhammaan cilladaha hidde-sideyaasha ee ku habboon dhammaan 79 hidde-sideyaasha hal baaritaan halkii ay si isdaba joog ah u ahaan lahaayeen. Tijaabadani aad bay faa'iido ugu leedahay ogaanshaha neoplasms-ka myeloid neoplasms iyo kala saariddeeda sida ku cad talada haga. Waxay ku siinaysaa macluumaadka saadaasha ee ku saabsan haddii bukaanku u baahan doono daawaynta caadiga ah ama daaweynta kemotherabi ee qiyaasta sare ama dhuuxa lafta ku-tallaalidda dawo joogto ah. Waxa kale oo ay gacan ka geysataa xulashada bukaannada daawaynta la beegsaday.

• Lymphoid neoplasms:

  Waxay ka caawisaa habaynta khatarta waxayna hagtaa daaweynta.