Molecular Pathology

Molecular Pathology yog ib thaj chaw ntawm pathology uas siv cov ntsiab lus, cov tswv yim thiab cov cuab yeej ntawm molecular biology uas yog ib qho tseem ceeb heev rau kev xyaum ntawm cov tshuaj precision.

Myeloid neoplasms

• Mob myeloid leukemia.
• Myeloproliferative neoplasms.
• Myelodysplastic syndromes.
• Myelodysplastic / myeloproliferative neoplasms.
• Myeloid/lymphoid neoplasms nrog eosinophilia thiab gene rearrangement.
• Mastocytosis.

Lymphoid neoplasms

• B-lymphoblastic leukemia/lymphoma
• Mob ntsws lymphocytic
• Plasma cell neoplasms
• High-qib B-cell lymphoma

Siv cov thev naus laus zis sib txawv xws li PCR lub sijhawm tiag tiag, Fluorescence hauv situ hybridization (FISH), Fragment analysis thiab Next Generation Sequencing (NGS) pab kom paub meej cov caj ces txawv txav ua rau tus kab mob.

• PCR tso cai rau kev nthuav dav ntawm cov seem ntawm DNA los pab nrhiav kom pom. PCR tuaj yeem siv los nrhiav qee qhov kev hloov pauv hauv cov noob caj noob ces lossis chromosome, uas tuaj yeem pab nrhiav thiab kuaj mob caj ces lossis kab mob, xws li mob qog noj ntshav.
• Lub sij hawm tiag tiag PCR pib nrog DNA / RNA template (extracted los ntawm tus neeg mob tus qauv) thiab lub hom phiaj ntawm kev txaus siab (xws li JAK2 kev hloov nyob rau hauv myeloproliferative neoplasms los yog BCR-ABL fusion transcripts nyob rau hauv CML) yog amplified ntau lab lub sij hawm, uas yuav kuaj tau nyob rau hauv lub sij hawm thiab tshaj tawm zoo (ntaus / tsis pom) lossis ntau (xws li luam / ml).
• FISH – molecular cytogenetics method/laboratory techniques for detecting and searching a specific DNA sequence on a chromosome, wherein the samples are processed to provide the binding of target DNA with the probe DNA labeled with fluorochrome, uas tso cai rau pom tom ntej los ntawm fluorescence microscope.
• Fragment tsom xam: Tom qab lub hom phiaj amplification (paub PCR), kev sib cais thiab kev pom ntawm fluorescently labeled fragments (DNA) raws li qhov loj me (xws li Microsatellite Instability).
• NGS (Tom ntej no sequencing) - Ua kom muaj kev tshawb nrhiav ib txhij ntawm kev kuaj mob thiab kev hloov pauv ntawm cov noob caj noob ces nyob rau hauv ntau cov noob rau cov hom phiaj kho mob. Qhov kev ntsuam xyuas pib nrog kev npaj tsev qiv ntawv thiab cov qauv tsim tau yog clonally amplified thiab enriched uas yog ntxiv sequenced. Kev hloov pauv caj ces yog sau tseg siv cov software muag khoom uas txuas nws mus rau cov kev kho mob muaj feem xyuam, kev sim tshuaj thiab kev pheej hmoo.

• Cov qog nqaij hlav:

  10ng ntawm cov tswv yim nucleic acid pom cov phiaj xwm ua tau thoob plaws 52 cov noob. Yog tias lub hom phiaj ua tiav tau raug txheeb xyuas, cov phiaj xwm kho tau muab, uas lub hom phiaj tshwj xeeb cov noob thiab cov proteins uas koom nrog hauv kev loj hlob thiab ciaj sia ntawm cov qog nqaij hlav cancer yam tsis muaj kev cuam tshuam rau lub cev ib txwm muaj. Nws ua haujlwm los ntawm kev thaiv cov cim kev loj hlob tseem ceeb rau kev loj hlob ntawm cov qog nqaij hlav cancer, ua kom nws txoj kev puas tsuaj thiab txo cov hlab ntsha tshiab uas xav tau rau nws txoj sia. Qhov kev kho no yuav ua kom ntev lub neej ntawm tus neeg mob thiab txhim kho tag nrho cov txiaj ntsig ntawm tus neeg mob. Pab hauv kev kuaj mob ntawm cov nqaij mos sarcomas nrog rau cov qog nqaij hlav me me los ntawm kev kuaj xyuas tus yam ntxwv cytogenetic abnormality.

• Myeloid neoplasms:

  Los ntawm Ib txhij kuaj pom txhua qhov cuam tshuam ntawm caj ces txawv txav thoob plaws 79 cov noob hauv ib qho kev ntsuam xyuas es tsis ua raws li qhov sib txuas. Qhov kev sim no muaj txiaj ntsig zoo rau kev kuaj mob ntawm myeloid neoplasms thiab nws cov kev faib tawm raws li cov lus qhia pom zoo. Nws muab cov ntaub ntawv pov thawj txog seb tus neeg mob puas yuav xav tau kev kho mob lossis tshuaj kho mob siab lossis kho pob txha pob txha kom kho tau mus tas li. Nws kuj pab xaiv cov neeg mob rau kev kho mob.

• Lymphoid neoplasms:

  Pab hauv kev pheej hmoo stratification thiab coj kev kho mob.