Molecular Pathology

Molecular Pathology ndi gawo lazachipatala lomwe limagwiritsa ntchito mfundo, njira ndi zida za biology ya mamolekyulu omwe ndi chida chofunikira kwambiri pakugwiritsa ntchito mankhwala olondola.

Myeloid neoplasms

• Acute Myeloid Leukemia.
• Myeloproliferative neoplasms.
• Myelodysplastic syndromes.
• Myelodysplastic/myeloproliferative neoplasms.
• Myeloid / lymphoid neoplasms ndi eosinophilia ndi kukonzanso jini.
• Mastocytosis.

Lymphoid neoplasms

• B-lymphoblastic leukemia/lymphoma
• Chronic lymphocytic leukemia
• Ma cell a plasma neoplasms
• B-cell lymphoma yapamwamba kwambiri

Kugwiritsa ntchito matekinoloje osiyanasiyana monga PCR yanthawi yeniyeni, Fluorescence in situ hybridization (NSOMBA), Fragment analysis ndi Next Generation Sequencing (NGS) zimathandiza kufotokoza bwino za kusakhazikika kwa majini komwe kumayambitsa matendawa.

• PCR imalola kukulitsa zidutswa za DNA kuti zithandizire kuzindikira. PCR ingagwiritsidwe ntchito kuyang'ana kusintha kwina mu jini kapena chromosome, zomwe zingathandize kupeza ndi kuzindikira zachibadwa kapena matenda, monga khansa.
• Nthawi yeniyeni PCR imayamba ndi template ya DNA/RNA (yotengedwa kuchokera ku zitsanzo za odwala) ndi zolinga za chidwi (monga kusintha kwa JAK2 mu myeloproliferative neoplasms kapena BCR-ABL fusion transcripts mu CML) zimakulitsidwa nthawi mamiliyoni ambiri, zomwe zingathe kudziwika mu nthawi yeniyeni komanso adanenedwa moyenerera (zazindikirika / sizinazindikirike) kapena kuchuluka (mwachitsanzo makope/ml).
• NSOMBA - njira ya mamolekyulu a cytogenetics/njira ya labotale yodziwira ndi kupeza DNA yotsatizana pa chromosome, momwe zitsanzo zimasinthidwa kuti zithandizire kumangika kwa chandamale cha DNA ndi kafukufuku wa DNA wolembedwa ndi fluorochrome, yomwe imalola kuwonera motsatira ndi maikulosikopu ya fluorescence.
• Kusanthula kwachidutswa: Pambuyo pakukulitsa chandamale (PCR yodziwika bwino), kulekanitsidwa ndikuwonera tizidutswa ta fulorosenti (DNA) kutengera kukula kwake (mwachitsanzo Kusakhazikika kwa Microsatellite).
• NGS (Kutsatizana kwa m'badwo wotsatira) - Imathandiza kuzindikira nthawi imodzi ya kusintha kwa majini okhudzana ndi matenda ndi ma genetiki mumitundu yambiri yomwe chithandizo chamankhwala chilipo. Kuyesa kumayamba ndi kukonzekera laibulale ndipo template yotsatiridwayo imakulitsidwa bwino ndikulemeretsedwa zomwe zimatsatiridwanso. Kusintha kwa ma genetic kumafotokozedwa pogwiritsa ntchito mapulogalamu omwe amapezeka pamalonda omwe amalumikizana ndi chithandizo chomwe chilipo, mayesero azachipatala komanso chiopsezo.

• Zotupa zolimba:

  10ng ya zolowetsa za nucleic acid zimazindikira zomwe zingatheke m'mitundu 52. Ngati chandamale chotheka chazindikirika, chithandizo chomwe chimayang'aniridwa chimaperekedwa, chomwe chimayang'ana majini ndi mapuloteni omwe amakhudzidwa ndikukula ndi kupulumuka kwa maselo a khansa popanda kukhudza maselo abwinobwino. Imagwira ntchito poletsa ma sign akukula ofunikira pakuchulukira kwa ma cell a khansa, imathandizira kuwonongedwa kwake komanso imachepetsa kupanga kwa mitsempha yatsopano yofunikira kuti ipulumuke. Chithandizochi chidzatalikitsa moyo wa wodwalayo ndikuwongolera zotsatira zonse za wodwalayo. Kumathandiza mu matenda zofewa minofu sarcomas komanso yaing'ono kuzungulira selo zotupa pozindikira khalidwe cytogenetic sali bwino.

• Myeloid neoplasms:

  Kuchokera Pamodzi amazindikira zolakwika zonse zamtundu wa 79 jini mu kuyesa kumodzi osati motsatizana. Kuyeza uku ndikothandiza kwambiri pakuzindikiritsa ma myeloid neoplasms ndi gulu lake monga momwe akulangizira. Limapereka chidziwitso chodziwikiratu ngati wodwalayo adzafunika chithandizo chokhazikika kapena mankhwala amphamvu kwambiri a chemotherapy kapena kumuika m'mafupa kuti achiritsidwe kwamuyaya. Zimathandizanso posankha odwala omwe akufuna chithandizo.

• Matenda a Lymphoid:

  Amathandizira pakuwongolera zoopsa ndikuwongolera chithandizo.