I-Molecular Pathology

I-Molecular Pathology

kokubonisana

I-Molecular Pathology

I-Molecular Pathology yinkalo ye isifo sengqondo esebenzisa imigaqo, ubuchule kunye nezixhobo zebhayoloji yemolekyuli, esona sixhobo sibaluleke kakhulu kunyango oluchanekileyo.

Iinkcazo

  • Iineoplasms ze-Myeloid
    • I-Acute Myeloid Leukemia.

    • Iineoplasms ze-Myeloproliferative.

    • Myelodysplastic syndromes.

    • I-Myelodysplastic/myeloproliferative neoplasms.

    • I-Myeloid/lymphoid neoplasms ene-eosinophilia kunye nohlengahlengiso lwemfuza.

    • Mastocytosis.

  • Iineoplasms ze-lymphoid

    • B-lymphoblastic leukemia/lymphoma

    • I-lymphocytic leukemia engapheliyo

    • Iiseli ze-plasma neoplasms

    • Umgangatho ophezulu we-B-cell lymphoma

Iinkqubo

Ukusebenzisa itekhnoloji eyahlukileyo efana ne-PCR yexesha lokwenyani, iFluorescence in situ hybridization (INHLANZI), uhlalutyo lweFragment, kunye ne-Next Generation Sequencing (NGS) inceda ukuchaza ngokuchanekileyo ukungahambi kakuhle kwemfuza okubangela isifo.

  • PCR ivumela ukwandiswa kwamaqhekeza e-DNA ukuba kube lula ukubhaqwa.I-PCR ingasetyenziselwa ukujonga utshintsho oluthile kumfuza okanye kwikhromozomu, enokunceda ekufumaneni nasekuxilongeni imeko yemfuza okanye isifo, esifana nomhlaza.

  • Ixesha le-PCR lokwenyani iqala nge-DNA/RNA template (ecatshulwe kwisampulu yesigulane), kunye neethagethi zomdla (umzekelo, ukuguqulwa kwe-JAK2 kwi-myeloproliferative neoplasms okanye i-BCR-ABL fusion transcripts kwi-CML) zandisiwe izihlandlo ezininzi zezigidi, ezinokubonwa ngexesha lokwenyani kwaye zixelwe. ngokomgangatho (ifunyenwe/ayifunyaniswanga) okanye ngokobungakanani (umz. iikopi/ml).

  • IFISH -indlela ye-molecular cytogenetics/indlela yelabhoratri yokukhangela kunye nokufumana ulandelelwano oluthile lwe-DNA kwichromosome. Iisampuli zicutshungulwa ukuququzelela ukubophelela kwe-DNA ekujoliswe kuyo kunye ne-probe ye-DNA ebhalwe nge-fluorochrome, evumela ukubonwa okulandelayo nge-fluorescence microscope.

  • Uhlalutyo lweqhekeza: Emva kokwandiswa kwethagethi (i-PCR eqhelekileyo), ukwahlukana kunye nokubonwa kwamaqhekeza abhalwe ngefluorescently (DNA) ngokusekelwe kubukhulu (umz. Ukungazinzi kweMicrosatellite).

  • I-NGS (Ulandelelwano lwesizukulwana esilandelayo) - Ivumela ukubonwa ngaxeshanye koxilongo kunye notshintsho olufanelekileyo lwemfuza kwimizila emininzi apho unyango ekujoliswe kulo iyafumaneka. Uvavanyo luqala ngolungiselelo lwethala leencwadi, kwaye ithempleyithi ephumayo ikhuliswa ngokudibeneyo kwaye ityetyiswe, ezilandelelaniswe ngakumbi. Utshintsho lwemfuza luchazwa kusetyenziswa isoftwe ekhoyo yorhwebo, eyinxulumanisa nonyango olukhoyo olufanelekileyo, ulingo lwezonyango kunye neenkcukacha zomngcipheko.

eziluncedo

  • Amathumba aqinileyo:
    • Ukusuka kwi-10ng yegalelo le-nucleic acid, ibona iithagethi ezinokuthathwa kuzo zonke iijini ezingama-52. Ukuba ithagethi esebenzayo ichongiwe, unyango olujoliswe kuyo lunikezelwa, olujolise kwiijini ezithile kunye neeprotheni ezibandakanyekayo ekukhuleni nasekuphileni kweeseli zomhlaza ngaphandle kokuchaphazela iiseli eziqhelekileyo. Isebenza ngokuthintela iimpawu zokukhula eziyimfuneko ekwandeni kweeseli zomhlaza, ikhawulezisa ukutshatyalaliswa kwayo kwaye inciphisa nokuveliswa kwemithambo yegazi emitsha efunekayo ukuze iphile. Olu nyango luya kwandisa ukusinda kwesigulane kunye nokuphucula umphumo wesigulane.
    • Inceda ekuxilongeni i-sarcomas yezicubu ezithambileyo kunye namathumba eseli amancinci angqukuva ngokufumanisa uphawu olungaqhelekanga lwe-cytogenetic.
  • I-Myeloid neoplasms:
    • Ngaxeshanye ibona zonke iziphazamiso zofuzo ezibandakanyekayo kuzo zonke iijini ezingama-79 kuvavanyo olunye kunokulandelelana. Olu vavanyo luluncedo kakhulu ekuxilongweni kwe-myeloid neoplasms kunye nokuhlelwa kwayo ngokwengcebiso yesikhokelo. Ibonelela ngolwazi lwe-prognostic malunga nokuba isigulane siya kufuna unyango oluqhelekileyo okanye i-chemotherapy yedosi ephezulu okanye ukutsalwa komongo wethambo unyango olusisigxina. Ikwanceda ekukhetheni izigulane unyango olujoliswe kuzo.
  • Iineoplasms ze-Lymphoid:
    • Inceda kwi-stratification yomngcipheko kunye nesikhokelo sonyango.